Possible Regulation of Larval Juvenile Hormone Titers in Bombyx mori by BmFAMeT6

Juvenile hormone (JH) plays a vital role in the growth, development, and reproduction of insects other arthropods. Previous experiments have suggested that BmFAMeT6 could affect duration silk moth’s larval stage. In this study, we established overexpression strain knockout using GAL4/UAS binary hybrid system CRISPR/Cas 9 system, respectively, found stage was shorter, while longer. Our results exhibited both JH titers BmKr-h1 levels larvae third instar were reduced significantly by overexpression, but increased obviously knockout. addition, injection farnesoic acid induced changes I II hemolymphs larvae. This study is first to directly reveal regulation insect relationship between (JH II). provides new perspective on regulating growth development such as Bombyx mori.